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KMID : 0624620120450020108
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BMB Reports
2012 Volume.45 No. 2 p.108 ~ p.113
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Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages
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Bae Deok-Sung
Kim Young-Hoon
Pan Cheol-Ho
Nho Chu-Won
Samdan Javzan
Yansan Jamyansan
Lee Jae-Kwon
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Abstract
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Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin E(2) (PGE(2)) production by LPS-stimulated Raw 264.7 cells, without a cytotoxic effect. Pre-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB).
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KEYWORD
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Cyclooxygenase-2, Cytokine, Inflammation, Macrophage, Nitric oxide, Protopine
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